Sex identification of wolf using non-invasive samples
We have developed new specific primers for sex determination from forensic samples of wolves (Canis lupus), such as hair, saliva, faecal, tooth and urine samples.
In order to improve molecular sexing, we perfomed a multiplex semi-nested polymerase chain reaction (PCR) and several replicated amplifications per sample to avoid errors in low quantity DNA samples, such as allelic dropout and false alleles.
The sex of individuals is automatically determined by capillary electrophoresis with a fluorescently labelled internal sex-specific primer form each pair.
Our mehtod yielded sex identification on 100% of invasive samples and 93% of forensic samples, being one of the highest success rates obtained from wild animals.
69.50 € per animal in blood or tissue samples
Blood or tissue samples. Please contact us for non-invasive samples analysis.
1. Download the form, print and sent with the samples
2. Send the samples with the printed form:
Servei Veterinari de Genètica Molecular Facultat de Veterinària. Universitat Autònoma de Barcelona 08193 Bellaterra (Barcelona). Spain
3. Original report will be send with the corresponding invoice
DNA samples are conserved during 2 years. SVGM guarantees the possibility of repeating the analysis during this time period to confirm the results when necessaryPayment instructions
Wire transfer (please convert payment to €) to Universitat Autonoma de Barcelona. Domestic and international personal account codes: IBAN ES52 2100 0424 3202 0018 6425 BIC/CODIGO SWIFT CAIXESBBXXX