Molecular analysis of PRNP gene in sheep (scrapie)
The name given to this infectious particle is prion. The host gene is called the prion protein (PrP) gene, which is a normal part of the genome of mammals and chickens. Its polypeptide product, called cellular PrP(superscript C), is a naturally-occurring protein attached to the outer surface of neurones and some other cells. PrP(superscript C) appears to play a role in maintaining the Purkinje cells of the cerebellum, which are essential for balance and muscular function.
The infectious agent, called scrapie PrP(superscript Sc), is a modifed form of PrP(superscript C). It is important to realise that these modifications involve no change in amino acid sequence. When PrP(superscript Sc) molecules enter a previously uninfected host, they convert the naturally occurring PrP(superscript C) molecules, produced by the host gene, into infectious PrP(superscript Sc) particles, which ultimately cause clinical signs in that animal, and which can spread to other animals, both horizontally (by infection) and vertically (by maternal transmission). (OMIA)
This disease has been recognised in sheep for many centuries. Extensive polymorphism has been discovered in codons 136, 154 and 171, giving rise to amino-acid substitutions at these sites. There is substantial evidence that at least some of this polymorphism is strongly associated with susceptibility/resistance to scrapie. Of note, the presence of arginine at codon 171 on both alleles of the PrP gene may confer resistance to the development of clinical TSE disease.
Codon 136: Val/Ala (GTC/GCC)
Codon 154: Arg/His (CGT/CAT)
Codon 171: Arg/Gln/His (CGG/CAG/CAT)
46.06 € (bulk rates are available). Please contact us
EDTA blood samples (1 ml). Samples should be refrigerated (4ºC) until shipment
1. Download the form, print and sent with the samples
2. Send the samples with the printed form:
Servei Veterinari de Genètica Molecular Facultat de Veterinària. Universitat Autònoma de Barcelona 08193 Bellaterra (Barcelona). Spain
3. Original report will be send with the corresponding invoice
DNA samples are conserved during 2 years. SVGM guarantees the possibility of repeating the analysis during this time period to confirm the results when necessaryPayment instructions
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